3895s  (Cell Signaling Technology Inc)

Journal: ACS Omega

Article Title: miR-205 Enhances Sensitivity to Genotoxic Agents in HNSCC Cells and Blocks Sphingosine Kinase 2 Action in Tumorigenicity

doi: 10.1021/acsomega.5c06726

Figure Lengend Snippet: miRNA-205 promotes radiosensitivity in SK2-expressing cell lines. (A) Orospheres formed on miR-205 overexpression in NOK-SK2 (pcDNA-miR-205) cells compared to control NOK-SK2 (pcDNA) post-UV exposure. Adhesion sphere assay (B); colony formation was determined by colony formation assay (C); and single spheres (D) on NOK-SK2 (pcDNA-miR-205) cells compared to control NOK-SK2 (pcDNA). (E) Western blot analysis for the protein levels of ZEB2, STAT3, p53Ac, and Snail. Images of spheres were obtained with a microscope. * p < 0.05, ** p < 0.01, *** p < 0.0001.

Article Snippet: Membranes were blocked with 5% nonfat dry milk (BioRad #1706404), incubated with primary antibodies against STAT3 (Cell Signaling #9139, 1:1000), phospho STAT3 (Cell Signaling #9145, 1:2000), NM23-H1 (Santa Cruz #sc-343, 1:1000), ZEB2 (Aviva #ARP39141_P050, 1:1000), p53Ac (Cell Signaling #2525, 1:1000), p53 (Cell Signaling #2527, 1:1000), SNAIL (Cell Signaling #3895, 1:1000), Calnexin (Cell Signaling #2433, 1:1000), and GAPDH (Cell Signaling #2118, 1:1000), overnight at 4 °C.

Techniques: Expressing, Over Expression, Control, Colony Assay, Western Blot, Microscopy